367 research outputs found
The dimensionality of self-rated employee performance : an integrated view
This study addresses the debate in the literature regarding the dimensionality of the job performance construct. The sample comprised 647 public servants from a state-based law enforcement organisation. Exploratory and confirmatory factor analyses delineate a four:factor structure, consisting of in-role behaviour, organisational citizenship behaviour directed towards (1) individuals or (2) the organisation, and a distinct latent variable deemed counter-productive work behaviour (CWB). The pattern of correlations among the four performance dimensions and between the performance dimensions and attitudes support the construct and discriminant validity of the four performance dimensions. Further, the .findings propose that CWB is a core, not discretionary, dimension of performance.<br /
Different DNA extraction methods can cause different AFLP profiles in grapevine (Vitis vinifera L.)
Amplified fragment length polymorphism (AFLP) is widely used for DNA fingerprinting and it has been broadly applied in population genetics. Since it is based on restriction digestion and PCR-based amplification it can be influenced by different chemical compounds commonly found in the isolated DNA. DNA extraction procedures may alter the AFLP banding profiles through DNA quality. Hence the DNA extraction method is crucial to produce reproducible AFLP-banding profiles.In this work two sets of AFLP analyses were performed on 62 Pinot noir, 6 Pinot blanc and 4 Pinot gris (Vitis vinifera L.) clones, and profiles obtained after three different DNA extraction methods were compared. AFLP profiles were different for the same genotypes due to the DNA extraction method used.
Mitralklappenendokarditis nach türkischem Opferfest
Zusammenfassung: Erysipelothrix rhusiopathiae ist der Erreger des Schweinerotlaufs. Systemische Infektionen durch E.rhusiopathiae sind eine Rarität, jedoch häufig (zu 90%) mit Endokarditiden verbunden. Ungefähr 60% der Endokarditiden entwickeln sich auf nicht vorgeschädigten Klappen, und trotz adäquater antibiotischer Therapie benötigen etwa ein Drittel der Patienten einen Klappenersatz. Wir beschreiben den Fall einer Hausfrau, die nach Zubereitung von Fleisch für das türkische Opferfest eine Mitralklappenendokarditis durch E.rhusiopathiae entwickelt
Application of current in situ hybridization techniques for grape phylloxera (Daktulosphaira vitifoliae, Fitch) and grapevine (Vitis spp. L.)
In situ hybridization and in situ PCR directly localize specific DNA and RNA sequences in tissues. To exactly focus on the processes occurring on cell- or tissue level, in situ techniques can be efficiently employed. Recent advances in viticultural research in the fields of genomics, proteomics and metabolomics are likely to employ these techniques to link DNA- or mRNA sequence information to physiological traits and processes occurring in the grapevine. In this paper, we present a range of possibilities for in situ techniques that can be applied in grapevine research. Two examples covering in situ PCR of grapevine roots and in situ hybridization of grape phylloxera will be given for illustration. Moreover, key steps of the techniques are discussed, which may be helpful to researchers aiming to employ in situ hybridization or in situ PCR.
Karyotype studies on grape phylloxera (Daktulosphaira vitifoliae FITCH)
A cytogenetic technique was developed to produce suitable chromosome spreads for phylloxera karyotype analysis. The karyotype for pathogenetic phylloxera was 2 n = 10. Karyotypes from haploid sex cells were found to vary between n = 5 and n = 6, the latter possibly indicating an aneuploidic aberration. Tetra- and polyploid cells were detected in somatic trophocytes. Preparation of phylloxera sex and somatic cells for chromosomal analysis reported here enables the study of genetic variation on a chromosomal scale
Histochemistry and anatomy of phylloxera (Daktulosphaira vitifoliae) nodosities on young roots of grapevine (Vitis spp).
Phylloxera (Daktulosphaira vitifoliae FITCH) induce galls (nodosities) on young grapevine roots. Histological and histochemical methods were applied to study the gall's morphology and enzyme activities (peroxidases, leucine aminopeptidases and acidic phosphatases). Susceptible V. vinifera cv. Cabernet Sauvignon was compared to the resistant rootstock 5 BB (V. berlandieri x V. riparia) using aseptic dual culture conditions. The gall induction phase was analyzed before visible signs of potential resistance responses were detected. Elevated metabolic activity has been found in nodosities compared to uninfected roots. Starch granule incorporation was detected in young galls and was highest at the feeding site. As galls mature, the starch density decreased at the feeding site and increased towards the periphery of the gall. Peroxidase, acidic phosphatase and leucine aminopeptidase activities were highest at the incision. No differences in enzyme activities could be detected between the two cultivars tested.
The importance of initial-final state correlations for the formation of fragments in heavy ion collisions
Using quantum molecular dynamics simulations, we investigate the formation of
fragments in symmetric reactions between beam energies of E=30AMeV and 600AMeV.
After a comparison with existing data we investigate some observables relevant
to tackle equilibration: dsigma/dErat, the double differential cross section
dsigma/pt.dpz.dpt,... Apart maybe from very energetic E>400AMeV and very
central reactions, none of our simulations gives evidence that the system
passes through a state of equilibrium. Later, we address the production
mechanisms and find that, whatever the energy, nucleons finally entrained in a
fragment exhibit strong initial-final state correlations, in coordinate as well
as in momentum space. At high energy those correlations resemble the ones
obtained in the participant-spectator model. At low energy the correlations are
equally strong, but more complicated; they are a consequence of the Pauli
blocking of the nucleon-nucleon collisions, the geometry, and the excitation
energy. Studying a second set of time-dependent variables (radii,
densities,...), we investigate in details how those correlations survive the
reaction especially in central reactions where the nucleons have to pass
through the whole system. It appears that some fragments are made of nucleons
which were initially correlated, whereas others are formed by nucleons
scattered during the reaction into the vicinity of a group of previously
correlated nucleons.Comment: 45 pages text + 20 postscript figures Accepted for publication in
Physical Review
Breakup Density in Spectator Fragmentation
Proton-proton correlations and correlations of protons, deuterons and tritons
with alpha particles from spectator decays following 197Au + 197Au collisions
at 1000 MeV per nucleon have been measured with two highly efficient detector
hodoscopes. The constructed correlation functions, interpreted within the
approximation of a simultaneous volume decay, indicate a moderate expansion and
low breakup densities, similar to assumptions made in statistical
multifragmentation models.
PACS numbers: 25.70.Pq, 21.65.+f, 25.70.Mn, 25.75.GzComment: 11 pages, LaTeX with 3 included figures; Also available from
http://www-kp3.gsi.de/www/kp3/aladin_publications.htm
A provisional database for the silicon content of foods in the United Kingdom
Si may play an important role in bone formation and connective tissue metabolism. Although biological interest in this element has recently increased, limited literature exists on the Si content of foods. To further our knowledge and understanding of the relationship between dietary Si and human health, a reliable food composition database, relevant for the UK population, is required. A total of 207 foods and beverages, commonly consumed in the UK, were analysed for Si content. Composite samples were analysed using inductively coupled plasma–optical emission spectrometry following microwave-assisted digestion with nitric acid and H2O2. The highest concentrations of Si were found in cereals and cereal products, especially less refined cereals and oat-based products. Fruit and vegetables were highly variable sources of Si with substantial amounts present in Kenyan beans, French beans, runner beans, spinach, dried fruit, bananas and red lentils, but undetectable amounts in tomatoes, oranges and onions. Of the beverages, beer, a macerated whole-grain cereal product, contained the greatest level of Si, whilst drinking water was a variable source with some mineral waters relatively high in Si. The present study provides a provisional database for the Si content of UK foods, which will allow the estimation of dietary intakes of Si in the UK population and investigation into the role of dietary Si in human health.<br /
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